The A46 Norton Disney near Lincoln was closed in both directions on Tuesday, February 3 after a horse box overturned on the carriageway.
Lincolnshire Police reported that at least one of the animals being transported was badly injured in the incident at around midday, and diversions were put in place.
Nottinghamshire Police also reported that they were working with Fire & Rescue officials and vets in order to free the horses.
Both horses were taken away from the scene for treatment and the Lincoln-bound carriageway was reopened at around 2pm.
The horses are recovering, the owners said.
A46 Lincoln bound reopened. Once recovery arrive Newark bound side will reopen. Both horses are being looked after thanks for patience.
— EMOpSS TRPT (North) (@TRPTNorth) February 3, 2015