B&Q closed its Lincoln store early on Thursday after a member of staff tested positive for coronavirus.
Workers were seen leaving the premises on Beevor Street, off Tritton Road, on Thursday afternoon and the shutters were pulled down.
The store has been closed for a deep clean, but it is expected to reopen again on Friday, October 9.
A spokesperson for B&Q said: “We expect the store to reopen Friday.
“We can confirm that our Lincoln store is temporarily closed following a team member, who has recently been on shift, receiving a positive COVID-19 test result.
“Closing the store for deep cleaning is a precautionary measure and goes beyond government advice.”