Road markings on the A180 between the Pyweipe and Lockhill roundabouts in Grimsby are to be repainted over the weekend.
North East Lincolnshire Council, in partnership with ENGIE, will give the markings a fresh coat of paint from Saturday, November 25.
Work is expected to be completed on Monday, November 27.
The existing red surfacing on Westgate roundabout, road markings and road studs will be refreshed.
To limit the effects of the works taking place, there will be a night-time road closure during this period, from 6pm to 6am the following morning.
A lane closure on Westgate roundabout will also be in place between 6pm and 6am, though access to Birchin Way, Pyewipe Road and Moody Lane will be maintained at all time.
North East Lincolnshire Council and its partner Engie would like to apologise for any inconvenience caused during the works.