Brayford Way bridge and Campus Way ramp will both need to close during the evenings for two weeks.
The work will replace the worn out carriageways and bridge joints and will start on Monday, April 6 from 8pm to 6am on weekdays.
Only Easter Friday and Easter Monday will be exempt from the closure.
Lincolnshire County Council has put in two separate diversion routes for northbound and southbound traffic.
The diversion route for northbound traffic will be from the University Roundabout through Ropewalk, the East-West Link Road, Cross Street, Portland Street, Pelham Bridge, Wigford Way, Newland and onto the Carholme junction.
Southbound traffic will follow through Newland, Wigford Way, Pelham Bridge, Kesteven Street, the East-West Link Road and onto the University Roundabout.
They will try to keep access to Campus Way through the Brayford Way bridge, but it may not be possible during certain works.
Campus Way residents have been told to find alternative parking locations to avoid problems getting vehicles in and out of the works zone after 7pm.
Councillor Richard Davies, the executive member for highways, said: “Not only will we be rebuilding the Campus Way ramp down to the mini-roundabout, but we’re also taking the opportunity to replace two of four bridge joints on the Brayford Way bridge.
“We know the bridge is one of Lincoln’s most used routes, so we’ll be doing everything we can to keep disruption to an absolute minimum.
“This includes carrying all works out overnight and working hard to complete the project earlier than planned.”