Lincoln trains will be replaced by coaches or cancelled altogether as workers plan to strike this weekend over a contract and pay dispute.
Train passengers are being advised to check the East Midlands Trains website for an amended timetable for Saturday, August 17.
It is the fourth strike action of its kind since last month and comes just days before a new a new company, Abellio, takes over the rail contract.
The Netherlands-based firm promised in April to replace existing trains with a cleaner, more reliable fleet with free WiFi as well as 80% more seats at peak times.
East Midlands Trains has a replacement coach service in place on the Peterborough through Lincoln and onto Doncaster line, which will be running in both directions all day.
RMT general secretary Mick Cash previously said: “East Midlands Trains’ lack of commitment to deal with our concerns over contractual issues such as compulsory Sunday working, wage discrimination and other issues is a total disgrace.
“As a result RMT, following a strong mandate, and after listening to our representatives, has been left with no alternative but to take strike action in order to make the company take the issues seriously.
“The union remains available for talks but no one should underestimate the resilience and determination of our EMT members to see justice.”